ABSTRACT
Objective:To explore the correlation between several single-nucleotide polymorphisms (SNPs) loci on the gene of human telomerase reverse transcriptase (hTERT)(including rs2736100、rs2853676) and the susceptibility to Hepatocellular carcinoma in Qingdao area.Methods:This case-control study included 165 diagnosed Hepatocellular carcinoma,195 with chronic hepatitis B and 402 Healthy people.Polymerase chain restriction fragment length polymorphism were performed to analyze the genotype of the loci on the gene of hTERT.Results:The frequency of TT genotype on rs2736100 were higher in the Hepatocellular carcinoma group compared to the control group、chronic hepatitis B and no HCC people(P＜0.05).The additive odds ratio of TT genotype for the risk of Hepatocellular carcinoma was 2.13、2.17 and 2.14.The frequency of AA genotype on rs2853676 were higher in the Hepatocellular carcinoma group compared to the control group、chronic hepatitis B and no HCC people (P＜0.05).The additive odds ratio ofTT genotype for the risk of Hepatocellular carcinoma was 3.42、2.39 and 3.02.Conclusion:There was a relationship between hTERT rs2736100 and rs2853676 polymorphism and susceptibility to Hepatocellular carcinoma.
ABSTRACT
<p><b>BACKGROUND AND OBJECTIVE</b>Invasion and metastasis are the most common causes of mortality for patients with colorectal neoplasms, and blocking invasion and metastasis in a timely fashion has become a hot research focus. We investigated the expression of the messenger RNA of Syndecan-1 and HPA-1 in colorectal cancer, and their correlation with invasion and metastasis.</p><p><b>METHODS</b>Real-time fluorescent quantitative polymerase chain reaction (PCR) was used to detect the expression of Syndecan-1 and HPA-1 in specimens from 49 patients with colorectal cancer, 49 paired adjacent colorectal neoplasms (2 cm from the carcinoma), and 49 surgical margins of paired normal colorectal mucosa tissue (5 cm from the carcinoma), to analyze their correlation with clinicopathologic characteristics of colorectal neoplasm.</p><p><b>RESULTS</b>The expression of HPA-1 mRNA was significantly higher in colorectal cancer (40.56 +/- 11.75) than that in the paired adjacent colorectal neoplasms (18.28 +/- 11.33) and normal colorectal mucosa tissue (10.80 +/- 10.20) (all P < 0.001). The expression of HPA-1 mRNA was significantly higher in paired adjacent colorectal neoplasms than that in normal colorectal mucosa (P < 0.05). The expression of Syndecan-1 mRNA was significantly higher in normal colorectal mucosa (61.21 +/- 12.96) than in the paired adjacent mucosa (14.35 +/- 11.06) or colorectal cancer (10.12 +/- 8.58) (all P < 0.001). The expression of Syndecan-1 mRNA was significantly higher in the paired adjacent mucosa than that in colorectal cancer (P < 0.05). The decreased expression of Syndecan-1 mRNA and the increased expression of HPA-1 were closely associated with the degree of differentiation, the depth of infiltration, lymph node metastasis, vessel metastasis, and TNM staging of colorectal cancer (all P < 0.05). Spearman rank correlation analysis demonstrated a significant correlation between Syndecan-1 and HPA-1(r = -0.405, P < 0.05).</p><p><b>CONCLUSIONS</b>The expression of Syndecan-1 mRNA was significantly highest in normal colorectal mucosa and the expression of HPA-1 mRNA was significantly highest in colorectal cancer. At the same time, the decreased expression of Syndecan-1 mRNA and the increased expression of HPA-1 mRNA can promote the invasion and metastasis of colorectal cancer. The determination of Syndecan-1 and HPA-1 may be of value in the treatment as well as in the prognosis of patients with colorectal cancer.</p>